Transgenic Mice Expressing Green Fluorescent Protein under the
Por um escritor misterioso
Last updated 17 julho 2024
![Transgenic Mice Expressing Green Fluorescent Protein under the](https://www.jneurosci.org/content/jneuro/23/18/7143/F6.large.jpg)
The melanocortin-4 receptor (MC4-R) is an important regulator of energy homeostasis, and evidence suggests that MC4-R-expressing neurons are downstream targets of leptin action. MC4-Rs are broadly expressed in the CNS, and the distribution of MC4-R mRNA has been analyzed most extensively in the rat. However, relatively little is known concerning chemical profiles of MC4-R-expressing neurons. The extent to which central melanocortins act presynaptically or postsynaptically on MC4-Rs is also unknown. To address these issues, we have generated a transgenic mouse line expressing green fluorescent protein (GFP) under the control of the MC4-R promoter, using a modified bacterial artificial chromosome. We have confirmed that the CNS distribution of GFP-producing cells is identical to that of MC4-R mRNA in wild-type mice and that nearly all GFP-producing cells coexpress MC4-R mRNA. For example, cells coexpressing GFP and MC4-R mRNA were distributed in the paraventricular hypothalamic nucleus (PVH) and the dorsal motor nucleus of the vagus (DMV). MC4-R promotor-driven GFP expression was found in PVH cells producing thyrotropin-releasing hormone and in cholinergic DMV cells. Finally, we have observed that a synthetic MC3/4-R agonist, MT-II, depolarizes some GFP-expressing cells, suggesting that MC4-Rs function postsynaptically in some instances and may function presynaptically in others. These studies extend our knowledge of the distribution and function of the MC4-R. The transgenic mouse line should be useful for future studies on the role of melanocortin signaling in regulating feeding behavior and autonomic homeostasis.
![Transgenic Mice Expressing Green Fluorescent Protein under the](https://www.thermofisher.com/antibody/images/650/A21311-GFP-WB-20210323110406.jpg?time=20220826)
GFP Polyclonal Antibody, Alexa Fluor™ 488 (A-21311)
![Transgenic Mice Expressing Green Fluorescent Protein under the](https://www.eneuro.org/content/6/5/ENEURO.0198-19.2019/F1.large.jpg)
Off-Target Effects in Transgenic Mice: Characterization of
![Transgenic Mice Expressing Green Fluorescent Protein under the](https://www.aveslabs.com/cdn/shop/products/gfp-if-2_800x.webp?v=1673304180)
Anti-Green Fluorescent Protein Antibody
![Transgenic Mice Expressing Green Fluorescent Protein under the](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs41598-020-71493-x/MediaObjects/41598_2020_71493_Fig1_HTML.png)
A novel protocol to detect green fluorescent protein in unfixed
![Transgenic Mice Expressing Green Fluorescent Protein under the](https://images.novusbio.com/images2/GFP%20Antibody-Immunohistochemistry-NB600-308-img0037.jpg)
GFP Antibody (NB600-308): Novus Biologicals
![Transgenic Mice Expressing Green Fluorescent Protein under the](https://ars.els-cdn.com/content/image/1-s2.0-S0925477398000938-gr6.jpg)
Generating green fluorescent mice by germline transmission of
Fluorescent green transgenic mice with GFP gene - Stock Image
![Transgenic Mice Expressing Green Fluorescent Protein under the](https://media.cheggcdn.com/study/221/221132e7-64d2-40a6-a345-d761c814999d/image.png)
Solved I created a transgenic mouse that had the green
![Transgenic Mice Expressing Green Fluorescent Protein under the](https://www.ptglab.com/products/pictures/webp/eGFP-Antibody-66002-1-Ig-WB-297076.webp)
GFP tag antibody (66002-1-Ig)
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